EasyPGX® ready KRAS RT021 (48 test, CE IVD)


Main features

Detection of the main mutations of exon 2 (codons 12, 13), of exon 3 (codons 59, 61) and of exon 4 (codons 117, 146) of the gene KRAS using 8
oligo mixes. Each mix allows the co-amplification of one or more mutated alleles plus an endogenous control gene. A specific oligo control mix enables
the evaluation of the quality and the quantity of the DNA in each sample.


The kit is provided with the following controls:

  • Positive control sample containing a mixture of synthetic DNA sequences that correspond to each mutation detected by this kit in a background of wild-type genomic DNA;
  • Negative control.


The kit allows the detection of low percentages of mutated allele in presence of high amounts of wild-type genomic DNA by real-time amplification with sequence-specific probes marked with FAMb and HEX. LOD down to 0.5%.

Starting material

The kit allows the analysis of DNA extracted from fresh, frozen, formalin-fixed paraffin-embedded (FFPE) tissues and plasma*.

*Please note that extraction from plasma is sold separately ( H8040)

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